Abstract: Objectives: Bone tissue grinding is one of the common and basic applications in orthopedic surgery clinics. The grinding process is energy-intensive and generates a lot of grinding heat. The accumulation of this heat may cause thermal damage to biological tissues. This paper presents experimental research to investigate the bone-grinding heat and the cooling method. Methods: The combined influence of nozzle position and feed direction on the cooling effect of bone grinding under cryogenic spray cooling conditions is experimentally investigated. A bone grinding platform with three-dimensional motion, as well as a cryogenic spray generation device, is designed and constructed. A spherical diamond grinding head with a diameter of 4 mm and a grit size of #150 is utilized. Fresh bovine cortical bone is used as the processing sample. The temperature at the nozzle outlet is 13 ℃, and the flow rate valve regulates the coolant flow rate to 400 mL/h. A three-dimensional force transducer (DJSW-40, China) is connected to a data acquisition system, which captures the forces applied to the bone sample along the X, Y, and Z directions at a frequency of 100 Hz. Simultaneously, a 0.1 mm diameter type K thermocouple (Omega Inc., TT-K-36) is embedded inside the bone sample to measure the grinding temperature in real-time. Three different nozzle arrangements were designed: above, in front of, and to the side of the abrasive tool, with the nozzles 10 mm away from the spray surface. Six sets of experiments (3×2) were designed using three nozzle orientations and two feeding directions. Each set of experiments was repeated three times to study the cooling effect of the spray under the combined influence of nozzle orientation and feed direction. Results: (1) During bone grinding, the abrasive tool is subjected to three orthogonal directional forces, namely F_X (the tangential grinding force used for removing material), F_Y (the axial grinding force, representing the resistance of the abrasive tool during its feed), and F_Z (the normal grinding force, which serves as the support force of the workpiece on the abrasive tool). For forward feed, the average values of the individual forces are: F_X = 0.37 N, F_Y = -0.72 N, F_Z = 1.38 N. For backward feed, F_X = 0.46 N, F_Y = 0.78 N, F_Z = 1.67 N. Since the grinding tool remains in the same rotational direction, the tangential force F_X is consistently positive. For forward/backward feed, the axial force F_Y is in the -Y and +Y directions respectively, thus the sign of the F_Y value changes. When feeding forward/backward, the tangential force (F_X) is 0.37 N and 0.46 N, respectively, which are relatively similar to each other, in accordance with the grinding theory. The power consumed for grinding is approximately 1.6 W and 1.9 W for forward and backward feed, respectively. (2) The nerve tissue is more heat-sensitive than bone tissue. Taking the human body's 37 ℃ as the base temperature, the threshold for the occurrence of thermal injury is 43 ℃, so the temperature rise threshold for thermal injury of nerve tissue is 6 ℃. In our experiment, the maximum temperature rise of bone under low-temperature spray cooling was lower than 4 ℃, indicating that the cooling method is effective. The effect of the nozzle arrangement was investigated under a fixed forward or backward feeding direction. When the abrasive tool is fed forward, the cooling of the thermocouple under the front nozzle is obvious. This is because, in addition to the contact arc area between the abrasive tool and the bone sample, a portion of the coolant from the front nozzle is sprayed onto the bone sample surface, resulting in a pre-cooling effect within the bone. When the abrasive tool is fed backward, the grinding temperature is lowest when the nozzle is placed above. For the different nozzle orientations, the side nozzles are in a perpendicular plane to the feed direction (Y-direction) of the grinding tool, so the feed direction has the least influence on the grinding temperature. The upper and front nozzles are in the same plane as the feed direction of the abrasive tool, so the influence of the feed direction is more significant. Conclusions: (1) The average tangential grinding force is 0.42 N, axial grinding force is 0.75 N, normal grinding force is 1.53 N, and the average power consumed by grinding is approximately 1.75 W when bone grinding is performed at a depth of 0.5 mm using a spherical diamond abrasive tool with a diameter of 4 mm. (2) Under the cooling effect of the cryogenic spray, the maximum temperature rise of grinding is less than 4 ℃, which can effectively prevent the occurrence of thermal damage in biological tissues. The temperatures of the two thermocouples in the same set of experiments were more consistent when the nozzle was placed above or side, while there was a significant difference in the temperatures of the two thermocouples when the nozzle was placed in front. This indicates that the cooling effect is more uniform when the nozzle is placed above and to the side. (3) The coupling of the nozzle arrangement and the feeding mode has a greater impact on the grinding temperature. When the nozzle is placed on top, it is favorable to backward feeding; when the nozzle is placed in front, it is conducive to forward feeding; and when the nozzle is placed on the side, there is no significant difference in the temperature between forward and backward feeding.