双层纳米孔的制造与应用

黄洁钰; 曾兆炜; 王成勇; 袁志山

doi:10.13394/j.cnki.jgszz.2023.0105

双层纳米孔的制造与应用

doi: 10.13394/j.cnki.jgszz.2023.0105

黄洁钰^{1, 2, 3, 4,},
曾兆炜^{1, 2, 3, 4,},
王成勇^{1, 2, 3, 4,},
袁志山^{1, 2, 3, 4, ,}

1.
广东工业大学机电工程学院，广州 510006
2.
广东工业大学，广东省微创手术器械设计与精密制造重点实验室，广州 510006
3.
高性能工具全国重点实验室（广东工业大学），广州 510006
4.
广东工业大学，智慧医疗创新技术中心，广州 510006

基金项目: 广东省自然科学基金（2021A1515012457）。

详细信息

作者简介:
王成勇，男，1964 年生，博士、教授、博士生导师。主要研究方向：高速加工涂层刀具制备、难加工材料的精密超精密与纳米加工理论与技术、超硬材料及其工具制造和应用技术。E-mail：cywang@gdut.edu.cn

通讯作者:
袁志山，男，1987 年生，博士、副教授、硕士生导师。主要研究方向：微纳制造技术与界面科学。E-mail：zhishanyuan@gdut.edu.cn

中图分类号: TH77
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出版历程
- 收稿日期: 2023-05-08
- 修回日期: 2023-05-27
- 录用日期: 2023-06-30
- 网络出版日期: 2024-06-28
- 刊出日期: 2024-06-28

Fabrication and application of double-layer nanopore

HUANG Jieyu^{1, 2, 3, 4
,},
ZENG Zhaowei^{1, 2, 3, 4
,},
WANG Chengyong^{1, 2, 3, 4
,},
YUAN Zhishan^{1, 2, 3, 4
, ,}

1.
Mechanical and Electrical Engineering, Guangdong University of Technology, Guangzhou 510006, China
2.
Guangdong Provincial Key Laboratory of Minimally Invasive Surgical Instruments and Manufacturing Technology, Guangdong University of Technology, Guangzhou 510006, China
3.
State Key Laboratory of High Performance Tools (Guangdong University of Technology), Guangzhou 510006, China
4.
Smart Medical Innovation Technology Center, Guangdong University for Technology, Guangzhou 510006, China

摘要

摘要: 由于具有孔径可调节、物理化学性质稳定、极端环境适应性强、集成度高等优点，固态纳米孔逐渐成为最具潜力的单分子测序工具。在固态纳米孔发展过程中，提高其单分子检测精度一直是研究人员关注的重点。近年来，双层纳米孔受到了广泛关注。与传统的单层纳米孔相比，双层纳米孔具有的孔-腔-孔结构提供了2个分子识别位点和纳米受限空间。双孔提供的2个分子识别位点可以在单次过孔事件中获得2次目标信号，所获得的双重检测信号不仅丰富了检测信息，也为信号分析提供了最为直接的对比信息源。此外，双层孔中的空腔还可作为单分子化学反应器。因此，双层纳米孔的出现拓宽了纳米孔传感器的应用范围，在单分子检测方面具有广阔的应用前景。本文概述了纳米孔的发展历程，并重点介绍了双层纳米孔的制造方法及其在单分子检测领域的应用。
- 固态纳米孔 /
- 双层纳米孔 /
- 双重检测信号 /
- 制造方法
Abstract: Solid-state nanopores have become the most promising single-molecule sequencing tools due to their adjustable pore size, stable physical and chemical properties, strong adaptability to extreme environments, and high integration. In the area of solid-state nanopores research, improving the detection accuracy of single molecules has always been the core concern of researchers. In recent years, the double-layer nanopores has attracted wide attention. Compared with traditional single-layer nanopore, the "pore-cavity-pore" structure of double-layer nanopores provides two molecular recognition sites and nanoconfined space. The two molecular recognition sites provided by the two nanopores can obtain two target signals in a single translocation event, which not only enrich the detection information but also provide the most direct comparison information source for signal analysis. In addition, the "cavity" in the double-layer nanopores can be used as a single-molecule chemical reactor. Therefore, the appearance of double-layer nanopores broadens the application range of nanopore sensors and has an important application prospect in single molecule detection. In this paper, the development history of nanopores is summarized, and the fabrication methods of double-layer nanopores and their applications in the field of single-molecule detection are emphatically introduced.
- solid-state nanopores /
- double-layer nanopore /
- double detection signal /
- fabrication methods

HTML全文

图 1 纳米孔检测原理

I₀-基准电流，ΔI-电流变化幅值，Δt-持续时间

Figure 1. Schematic diagram of nanopore detection principle

I₀-Reference current，ΔI-Current variation，Δt-Lasting time

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图 2 孔-腔-孔结构^[33]

Figure 2. Pore–cavity–pore structure^[33]

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图 3 纳米孔熵笼结构^[32]

Figure 3. Nanopore entropic cage structure^[32]

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图 4 氮化硅-石墨烯双层孔结构^[35]

Figure 4. Double-layer nanopore consisting of silicon nitride and graphene^[35]

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图 5 纳米孔阵列膜-纳米孔结构^[36]

Figure 5. Arrayed nanopore membrane - nanopore structure^[36]

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图 6 堆叠孔-腔-孔结构研究λDNA分子的电泳飞行时间^[30]

Figure 6. Time-of-flight of λDNA molecule using stacked ‘pore-cavity-pore’ device^[30]

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图 7 纳米熵笼作为单分子化学反应器^[32]

Figure 7. Entropic cages as reactors for chemically modifying single DNA molecules^[32]

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图 8 双层纳米孔辅助DNA动力学校对^[31]

Figure 8. Application of stacked pore–cavity–pore device^[31]

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图 9 平面孔-腔-孔结构的应用

Figure 9. Application of in-plane pore–cavity–pore device

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参考文献(40)

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留言板

双层纳米孔的制造与应用

doi: 10.13394/j.cnki.jgszz.2023.0105

作者简介:
王成勇，男，1964 年生，博士、教授、博士生导师。主要研究方向：高速加工涂层刀具制备、难加工材料的精密超精密与纳米加工理论与技术、超硬材料及其工具制造和应用技术。E-mail：cywang@gdut.edu.cn

通讯作者:
袁志山，男，1987 年生，博士、副教授、硕士生导师。主要研究方向：微纳制造技术与界面科学。E-mail：zhishanyuan@gdut.edu.cn

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Fabrication and application of double-layer nanopore

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留言板

双层纳米孔的制造与应用

doi: 10.13394/j.cnki.jgszz.2023.0105

作者简介: 王成勇，男，1964 年生，博士、教授、博士生导师。主要研究方向：高速加工涂层刀具制备、难加工材料的精密超精密与纳米加工理论与技术、超硬材料及其工具制造和应用技术。E-mail：cywang@gdut.edu.cn

通讯作者: 袁志山，男，1987 年生，博士、副教授、硕士生导师。主要研究方向：微纳制造技术与界面科学。E-mail：zhishanyuan@gdut.edu.cn

计量

出版历程

Fabrication and application of double-layer nanopore

计量

出版历程

目录

作者简介:
王成勇，男，1964 年生，博士、教授、博士生导师。主要研究方向：高速加工涂层刀具制备、难加工材料的精密超精密与纳米加工理论与技术、超硬材料及其工具制造和应用技术。E-mail：cywang@gdut.edu.cn

通讯作者:
袁志山，男，1987 年生，博士、副教授、硕士生导师。主要研究方向：微纳制造技术与界面科学。E-mail：zhishanyuan@gdut.edu.cn